siRNA can be delivered into cells by infection of the cells with Lentiviral particle carrying a siRNA
gene. The gene is then integrated into chromosome DNA of the host cells and is constantly expressed
to achieve long term gene silence (>30 days). Lentiviral particles can be transducted into a wide
variety of cells. The major advantage of Lentiviral shRNA over regular shRNA is that vshRNA can be
delivered into both dividing and non-dividing cells to achieve stable long term gene silence effect. For
more comparison of siRNA, shRNA and vshRNA, please click here
The procedure of vshRNA preparation is shown in following flow chart:
Construction of a vshRNA vector and preparation of viral particles are two essential steps to achieve gene silence by vshRNA. EZBiolab provide both of the services.
Custom Construction of Lentiviral shRNA Vector
This service is for the projects that already have a sequence of shRNA. If you need guaranteed results
from our design, please see our TripleStrike package.
Construction of vshRNA vector is to insert a synthetic DNA oligos (60-110 bp) into a vector. This seemly simple experiment is much more troublesome than many expected for the following reasons:
If you already have a shRNA sequence , we can construct the vshRNA vector for you. This could save you a lot of time and troubles to make the construction yourself. We provide:
Guarantee to silence the target gene
Similar to our siRNA synthesis and regular shRNA vector construction services, our TripleStrike Custom vshRNA Vector Construction package provides design and construction of three vshRNA vectors targeting three different regions of a gene and a control shRNA vectors. Customer only need to provide us a gene accession number ( gene bank ID) or a gene sequence. We guarantee you that this gene will be silenced.
|Package Includes Design and Construction|
|1. Three shRNA expression vectors|
|number of shRNA vectors||3|
|target gene||customer assign|
|2. Negative control shRNA|
|number of shRNA dvectors||1|
|A gene bank ID (accession number) or the sequence of a gene and a blank vector
(customers can also provide three shRNA sequences of their own design)
|At least one of the three shRNAs will generate 70% silence effect at mRMA level
(no guarantee is offered for customer's own design)
|Delivery Time: -4 weeks|
The service includes construction of vshRNA vectors and preparation of viral particles.
The high titer of vshRNA lentiviral particles customer received are ready to infect host
cells and to silence the target gene.
For available promotors and report genes, please click here
Custom Preparation of vshRNA Lentivial Particle
If you already have a vshRNA vector, we can preparae viral particles for you. If you do not have the vector, we can help you to design a shRNA sequence, construct a vshRNA vector and then prepare viral particles. For detail and price, please email us for a quote
TripleStrike Custom vshRNA Lentiviral Particle Preparation Package
Guarantee to silence the target gene!
|Package Include||1. siRNA sequence design
2. vshRNA vector construction
3. shRNA Lentiviral particle preparation
|Customer Receive||1. Three vshRNA Lentiviral particles targeting three different regions of a gene
2. Three vshRNA vectors
|Customer Provide||The sequence of the target gene or its gene bank ID (accession number) and
|Guarantee||At least one of the three shRNAs will generate >70% silence result at mRNA
|Deliver Time||6-8 weeks|
|Package Include||vshRNA-TP-1 plus a large preparation of vshRNA viral particles (2 x 10^8 TU).
The sequence of the large prep is based on gene silence results of vshRNA-TP-1