the expressed shRNA is processed into siRNA and then blocks its target gene. Comparing to chemically synthesized siRNA, shRNA has these features:
Construction of shRNA vector is to insert a synthetic DNA oligos (60-110 bp) into a vector. This seemly simple experiment is much more troublesome than many expected for the following reasons:
this labour-intensive and time consuming work. Our service provides
This all-inclusive package provides design and construction of three shRNA vectors targeting three different regions of a gene and two control shRNA vectors. Customer only need to provide us a gene accession number ( gene bank ID) or a gene sequence. We guarantee you that this gene will be silenced by the package
Package Includes Design and Construction | |
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1. Three shRNA expression vectors | |
number of shRNA vectors | 3 |
target gene | customer assign |
quantity | 20 ug/clone |
purity | transfection grade |
2. Positive control shRNA | |
number of shRNA dvectors | 1 |
target gene | GAPDH |
quantity | 20 ug |
purity | transfection grade |
3. Negative control shRNA | |
number of shRNA dvectors | 1 |
target gene | NC |
quantity | 20 ug |
purity | transfection grade |
Customer Provide | |
A gene bank ID (accession number) or the sequence of a gene (customers can also provide three shRNA sequences of their own design) |
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Guarantee | |
At least one of the three shRNAs will generate 70% silence effect at mRMA level (no guarantee is offered for customer's own design) |
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Price: Email us for a quote | |
Delivery Time: 3-4 weeks |
Service Code | Construction | Time | Price ($)* |
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Rsh-01 | shRNA vector of customer provided shRNA sequence | 3-4 weeks | Email us for a quote |
Rsh-01p | Rsh-01 + positive control shRNA vector (20 ug) + negative control shRNA vector (20 ug) + RT PCR primer for positive control (0.5 OD) |
Email us for a quote | |
Rm-01 | shRNA vector of customer provided miRNA sequence | 4-5 weeks | Email us for a quote |
Rm-01p | Rm-01 +negative control miRNA vector (20 ug) +RT PCT primer for posive control (0.5 OD) |
4-5 weeks | Email us for a quote |
Promoter | H1 or U6 |
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Report gene | GFP |
Resistance | Hygromycin or Neomycin |
shRNA insertion | 25(stem) + 4 (loop) structure, for 25 bp target micro-30 miRNA |