the expressed shRNA is processed into siRNA and then blocks its target gene. Comparing to chemically synthesized siRNA, shRNA has these features:

• Transfection efficiency can be monitored by report genes
• Transfected cells can be selected from non-transfected ones based on antibiotic resistance
• Experiments are operated at DNA level, which is much less accessible to degradation.
• One time construction, unlimited supply.

For more comparison between siRNA and shRNA, click here

Construction of shRNA vector is to insert a synthetic DNA oligos (60-110 bp) into a vector. This seemly simple experiment is much more troublesome than many expected for the following reasons:

• synthesis of long oligos (>50mer) is prone to errors
• oligo for shRNAs often forms hairpin structure, which is difficult to be cloned into vector
• hairpin structure is difficult to be sequenced

this labour-intensive and time consuming work. Our service provides

• a large collection of EZBiolab's vectors specifically designed for shRNA
• multiple selections of report genes, promoters and antibiotic resistance
• free design service
• guaranteed sequence accuracy
• TripleStrike shRNA package that guarantees to knockdown your gene!

TripleStrike Custom shRNA Construction Package
Silence Result Guaranteed

This all-inclusive package provides design and construction of three shRNA vectors targeting three different regions of a gene and two control shRNA vectors. Customer only need to provide us a gene accession number ( gene bank ID) or a gene sequence. We guarantee you that this gene will be silenced by the package

Package Includes Design and Construction
1. Three shRNA expression vectors
number of shRNA vectors	3
target gene	customer assign
quantity	20 ug/clone
purity	transfection grade
2. Positive control shRNA
number of shRNA dvectors	1
target gene	GAPDH
quantity	20 ug
purity	transfection grade
3. Negative control shRNA
number of shRNA dvectors	1
target gene	NC
quantity	20 ug
purity	transfection grade
Customer Provide
A gene bank ID (accession number) or the sequence of a gene (customers can also provide three shRNA sequences of their own design)
Guarantee
At least one of the three shRNAs will generate 70% silence effect at mRMA level (no guarantee is offered for customer's own design)
Price: Email us for a quote
Delivery Time: 3-4 weeks

shRNA Vector Construction Service

Service Code	Construction	Time	Price ($)*
Rsh-01	shRNA vector of customer provided shRNA sequence	3-4 weeks	Email us for a quote
Rsh-01p	Rsh-01 + positive control shRNA vector (20 ug) + negative control shRNA vector (20 ug) + RT PCR primer for positive control (0.5 OD)		Email us for a quote
Rm-01	shRNA vector of customer provided miRNA sequence	4-5 weeks	Email us for a quote
Rm-01p	Rm-01 +negative control miRNA vector (20 ug) +RT PCT primer for posive control (0.5 OD)	4-5 weeks	Email us for a quote

• We provide free design service.
• For guaranteed knockdown, please see TripleStrike package

Vector and Insertion Options

Promoter	H1 or U6
Report gene	GFP
Resistance	Hygromycin or Neomycin
shRNA insertion	25(stem) + 4 (loop) structure, for 25 bp target micro-30 miRNA