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Yeast, Insect (baculovirus) and Mammalian Expression System Guaranteed yield and purity
For proteins difficult to be obtained in active form from E. Coli, we provide yeast, insect or mammalian cell cultures for protein expression.
Host Cell Selection Various factors determine if yeast, insect or mammalian is the best system for the expression of a target protein. These include post translational modification, source of the protein, the history of expression and purification and cost, affinity tag, yield , purity and available vectors and genes. In the term of cost, the order is Yeast < Insect < Mammalian. The order of yield is reverse.
Small Scale Test Run Once th host cell is decided, expression will first be tested in different cell stains to optimize expression level of the target protein. If the expression level is too low to yield sufficient amount of protein or the target protein fails to be expressed in biologically active form, the results will be reported to customer and the suggestion to alternative plans will be proposed.
Protein Production, Research Scale or Large Scale Research scale: 1 liter of cell culture Large Scale: 5, 10 20 and up to 50 liter of culture
Please email us for a quote.
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