Protein Expression and Purification Service
Fractional cost, Total solution
    Protein expression and purification are labor intensive and time consuming. To have
    sufficient amount of proteins at a desirable purity is often the bottleneck of a research
    project that ranges from structural biology to drug discovery.
EZBiolab protein expression and purification service can help you to solve this problem. Our price is very
affordable and our highly knowledgable scientists will work with you to meet your specific requirement.  
Combined with our codon optimization, gene synthesis, vector construction and protein refolding service,
we provide a total solution for protein production at a fractional cost
TOTAL SOLUTION
Services
Proteins
Systems
Scales
.> Expression
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.> Purification
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.> Refolding
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.> Characterization
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.> Target gene synthesis
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.> Vector construction
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.> Mutagenesis
.> Affinity tagged proteins
y
.> Non-tagged proteins
y
.> Aggregated proteins
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.> Membrane proteins
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.> Difficult proteins
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.> 15N/13C labeled proteins
y
.
.> Bacteria
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.> Yeast
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.> Insect
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.> Mammalian
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.> In vitro translation
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.
.
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> Regular scale for
....research

.> Large scale for
....industry application
Service Highlight
EZBiolab
Setting High Standards for
Scientific Excellence
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Contact Us: service@ezbiolab.com
Product and Service

..Portein Expression and
nPurification
....> E.Coli system
....> Yeast, Insect and mammalian
....> Protein refolding
....> Protein characterization
....> 13C/15N labeling
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..Peptide Synthesis
.......
........Antibody Development
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..siRNA Preparation
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..Gene Synthesis
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..Assay development  
Quote and Order

..Request a quote
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..Order by email
..Contact: service@ezbiolab.com
A Low Cost Solution to Protein Supply
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Protein expression and purification is often to be the most difficult step for a research project in term of
time and labour involved. Our price is so affordable that  it is no longer worth for you to spend your
valuable time in a cold room.
For projects that require reliable and  affordable protein supply over long time, we
provide additional discount that will further reduce the cost substantially:
u
> 20% off for repeat orders of the same protein. This discount will be increased to
...30% starting from the 3rd  order of the same protein
y
> 15% off for mutant proteins of the original protein*. Start from the 2nd order, the . .
,,,same mutant will also enjoy 30% discount.
y
m* The discount does not apply to mutants that require a new purification scheme
nmdue to the change of protein properties caused by mutation
Finally, I can afford a
break from cold rooms
Sophisticated Technology, Different Results
Instead of brutal force approach, we combine advanced technology with our in-depth knowledge on protein
sciences to offer a comprehensive and sophisticated solution. This is reflected by the broad range services
we offer. Some of them are uniquely found from EZBiolab.  Our results are reliable, analysis is in-depth and
delivery is on-time.
Request a Quote
Our service is milestone based. Please email us the basic information of your project (sequence, amount
and purity required) as well as any other information such as if you already have a construction, the
solubility and stability of the target protein and if have, the references about expression and purification of
the target proteins.
We will return you a detailed experimental plan with a price quote.
EZBiolab Inc. All Right Reserved
Bacterial Expression System
Bacterium, mainly E. Coli is the most efficient protein expression system in term of yield and cost.  We find
that ~ 80% of proteins, regardless their origins, can be expressed and purified from
E. Coli in active form.
The key to our success is our decades experiences in expression condition optimization and  refolding
technology. Except those with specific modification critical to their functions, such as  glycosylation, most
proteins should be first tested in
E. Coli for its unmatched yield and low cost
                                                                                                                                                  detail

Yeast, Insect and Mammalian Expression Systems
.Some proteins, such as those with specific post-translational modification, multi-domain or multi-subunit
proteins requiring special assembling mechanism in a particular type of cells are impossible or very difficult
to be obtained in active forms from
E. Coli cells. For them, we provide protein expression and purification
service  from yeast, insect or  mammalian cells.
                                                                                                                                                 
 detail

Protein Refolding
Refolding is the key to proteins expressed in E. Coli with high yield but forming aggregated inclusion
bodies. Our sophisticated refolding technology including dynamic light scattering, CD and fluorescence
spectrum to characterize refolded proteins
                                                                                                                                                 
 detail

13C/15N labeling
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13C/15N labeled proteins are essential samples for NMR and MS studies. We provide custom preparation
of either uniformly labeled or site-specific labeled protein samples.
                                                                                                                                                   
detail

Protein Characterization
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> Molecular weight determination
> Purity determination
> Stability determination: absolute stability or relative stability
> Protein structure by CD
> Aggregation/oligomeration
> Binding
                                                                                                                                                    
detail