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Protein Characterization
EZBiolab provides research service to characterize some essential properties of protein
Protein Molecular Weight
Methods:
1. Gel electrophoresis
2. Size exclusive column
3. Mass Spec
Protein Sample Purity
Methods:
1. Gel electrophoresis
2. Western blot
3. Column chromatograph (HPLC)
Protein Stability
1. Free energy change of protein unfolding, dG and equilibrium constant of a protein between the fold and
the unfolded states in solution. This can give an estimation on how much a protein can resist denaturation
conditions such as temperature and urea concentration. It's also a reliable parameter to compare the
stability of two proteins. We call it absolute stability
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2. Stability of a protein under practical conditions. This measures changes of a protein structure and
function with time under a given condition. It determines half life of an active protein. We call it relative
stability.
Protein Aggregation
Some proteins form aggregated oligermer but is still soluble. As a result, this aggregation can not be
detected by visual inspection. We offer two methods to determine protein aggregation:
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1. Size exclusive chromatograph
2. Dynamic light scatter (DLS)
Protein Structure Determination By CD (Circular Dichroism)
CD is a commonly used method to determine structure of a protein. It determines
1. Secondary structure, particular alpha helix content in a protein
2. If a protein is unfolded.
3. Protein stability
Protein-Ligand Binding and Protein-Receptor Binding
We provide research service to determine protein-ligand interaction. Our main approach is fluorescence,
including fluorescence intensity and fluorescence anisotropy (polarization). We determine Kd, Ki (based on
competition assay) and ICk50 of binding. Our in-depth knowledge and extensive experience in both data
analysis and fluorescence methodology guarantee you a publication quality result.
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EZBiolab
Setting High Standards for
Scientific Excellence
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Contact Us: service@ezbiolab.com
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To request a quote for one of above services, please email us your project to service@ezbiolab.com.
We will return you with a price quote including detailed experimental plan, data analysis method and the
expected results.
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