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Custom Polyclonal Antibody Production



  • Various packages to meet different needs.
  • Phosphor-specific and other modification-specific antibodies
  • Application guaranteed antibody
  • Free antigen design

Service Summary

Service Catalog # Package Price ($)


Regular Antibody
AB203 Complete Anti-Peptide Antibody
(rabbit or mouse)
Email us for a quote
AB202 Anti-Peptide Antibody
(rabbit or mouse)
Email us for a quote
AB201 Anti-Protein Antibody (rabbit or mouse) Email us for a quote

Site Specific Antibody
SAB01 Affinity Purified Anti-Phosphorylation Specific Antibody Email us for a quote
SAB02 Affinity Purified Other Site Specific Antibody Email us for a quote
Application Guaranteed Antibody AGW Western Blot Guaranteed Antibody Email us for a quote
AGP Immuno Precipitation Guaranteed Antibody Email us for a quote


Antibody Purification
AP100 Partial Purification
(ammonium sulfate precipitation)
           FREE
AP101 Protein A affinity Purification Email us for a quote
AP102 Peptide Antigen Affinity Purification Email us for a quote

Service detail

AB203                                                      Complete Anti-peptide Antibody Package
This package uses a peptide as antigen. The sequence of the peptide is derived from the target protein. This is the most popular package because it only requires target protein sequence to generate its antibody. The critical part is antigen design. We provide free assistance for antigen design
Animal:
  • 2 rabbits or 5 mice
Package includes:
  • Peptide synthesis (up to 15 aa)*
  • A Cys will be added to one end of the peptide and the other end will be blocked
  • Conjugation of peptide to carrier protein (KLH or others)
  • Immunization of animals
  • Partial purification of antibody
Customer receives :
  • 3-5 mg peptide antigen (unconjugated)
  • 1-3 ml pre-immune serum aliquots
  • Partial purified antibody from 30-50 ml antiserum of each rabbit by ammonium sulfate precipitation method. For antigen affinity or protein A purification, please see antibody purification
  • ELISA results and product certificate of analysis
Delivery time:
  • 11-13 weeks
Customer supplies: Note:
  • The peptide purity is guaranteed to be >70%. For higher guaranteed purity or longer than 15mer, price is slightly higher
  • The package is for standard protocol. The extended protocol is also available
AB201                                                              Anti-Protein Antibody Package
The package is same as AB203 except that it uses a purified protein as the antigen
Customer supplies:
  • Protein antigen: 1-5 mg with suggested purity >90%. The sample can be either purified protein or SDS gel strip. For purified protein, it should be submitted as lyopholized powder. For gel strip, extra charge applies ($75). Protein sample with <90% purity is also acceptable at the cost of reduced antibody quality, mainly specificity.
AB202                                                                Anti-peptide Antibody Package
If you already made a synthetic peptide and is going to use it as the antigen, this is the package for you. The package is same as AB203 except that customer providers the synthetic peptide. We do conjugation and immunization.
Customer supplies:
  • Antigens : > 5 mg of the peptide (>70% purity). Peptide should be sent as lyophilized powder
SAB01                                                      Complete Phospho-specific Antibody Package
This package delivers an antibody that recognizes a sequence with a phosphorylation site. The same sequence with de-phosphorylation will not be recognized.
Animal:
  • 2 rabbits or 5 mice
Package includes:
  • Synthesis of a phosphorylated peptide (up to 15 aa and purity >90%) and a non-phosphorylated version of the same peptide (purity >90%)
  • Conjugation of the phodpho-peptide to a carrier protein (KLH or others)
  • Immunization of animals
  • Antibody purification by two affinity columns: phospho and non-phospho peptides. The purified antibody is highly specific. It only recognizes phosphorylated site.
Customer receives :
  • 1-3 mg of each peptide (un-conjugated)
  • 1 ml pre-immune serum aliquots
  • Affinity purified anti-phospho-peptide specific antibody that only react s with phosphorylated site
  • ELISA results to both peptides and product certificate of analysis
Delivery time:
  • 12-14 weeks
Customer Supplies:
  • Peptide sequence
SAB02                                                 Site Specific Antibody Package
This package produces an antibody that only recognize a specifically modified site. Peptide with the same sequence but without the modification will not be recognized. The modification can be methylation, acetylation or others. The development strategy is similar to phosphor specific antibody (see SAB01). Please email us for a quote
AGW                                                    Western Guaranteed Antibody Package
Antibodies show high ELISA titer to a peptide antigen sometimes do not work in Western blot, IP and other experiments (please see our antigen design section for detail discussion about this). Our Western guaranteed antibody service eliminates this risk. You pay 0 if the antibody does not work for Western.
Package Include
  • Antigen design, peptide synthesis and conjugation, immunization and Western blot detection. Alternatively, customer can do the test by them own.
Customer Provide
  • Antigen protein samples for Western experiments. If a customer decide to do test himself, it is not necessary to provide us the antigens.
Guarantee
  • Western for endogenous and over expressed the target protein. If the antibody failed to detect the target protein by Western, we will either fully refund you or re-make the antibody for you, whichever you prefer. Please note the guarantee is only apply to the project that antigen designed and synthesized by EZBiolab.
AGP                                                        IP Guaranteed Antibody Package
This package offers immuno-precipitation guaranteed antibody
Package Include
  • Antigen design, peptide synthesis and conjugation, immunization and IP test. Alternatively, Customer can do the test them own.
Customer Provide
  • see Western Guaranteed Antibody Package
Guarantee
  • IP for over expressed target protein. If the antibody failed to precipitate the target protein by Western, we will either fully refund you or re-make the antibody for you, whichever you prefer. To request a quote, please email us antibody@ezbiolab.com