MAB203 Complete Anti-peptide Monoclonal Antibody Package
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Total time: 5-6 month
Total price: $4,285.00
Customer provide: A peptide sequence (free antigen design assistant is available)
Customer receive: Two vials each of the selected hybridomas producing antigen-specific antibodies
,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,together with 3-5 ml ascites and 3-5 mg peptide
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The service is broken into 4 phases. The result of each phase will be emailed to customer to allow you
make selections and decide if to proceed to the next phase.
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1. Peptide synthesis and conjugation, 490 (~3 weeks)
Peptide length: 15 residues including the Cys for KLH conjugation ( A Cys will be added to either end of the
peptide and the other end will be blocked)
Peptide purity: >90%;
Peptide quantity: 15-20 mg
Conjugation: Cys method. Other methods are also available.
Carrier protein: KLH. Other carriers such as BSA and OVA are also available upon request.
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2. Immunization and ELISA screening, $895 ( ~6 weeks)
5 Balb/c mice are immunized and boosted according to the standard immunization protocol. Immunization
may continue longer if response is not optimal. Tail bleeds are screened by ELISA ( Western blot and other
screening methods are available upon request. For details, please click here )
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3. Fusion and screening of specific clones, $1,635 ( ~6 weeks )
Once an acceptable titer is obtained, hybridoma fusion will be performed using splenocytes from the
mouse showing the best titer. The hybridoma cells are expanded and screened by ELISA to select clones
that show the best titers ( Western blot and other options are available. Please click here for the detail)
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4. Subcloning , $1,265 (~7 weeks)
Two clones selected by the customer will be subcloned three times by limiting dilution. Isotypes of all
selected clones will be determined.
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5. Delivery and report
2 vials each of the selected hybridomas producing antigen-specific antibodies together with 3-5 ml ascites
will be delivered to customer.
The final report includes the data of titer for the monoclonal antibodies, ELISA > 1:5000 (protein antigen)
or >1:2000 (peptide antigen).
Additional Services:
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Antigen design
free (about antigen design)
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Large scale monoclonal antibody production
For large scale production of a monoclonal antibody developed either by us or in your own lab, please email
us for a quote. We can produce them from cell culture or from accites.
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Antibody purification
1. Protein A column
Instead of ascites, the final delivery can be the antibody purified by a protein A column
Price: $150/clone
for purification of large amount of antibodies produced by cell culture method, please email us for a quote.
2. Peptide affinity column
$385.00/clone
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Additional clones in Phase III
$150.00/clone
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Antibody labelling
Biotin or fluorescence (FITC) labeling service is available. Please email us for a quote
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