Gene cloning Email us for a quote
The sequence of the gene to be
cloned and the template source,
such as a cDNA library
Design and synthesize primers
PCR and ligate PCR products into
our standard vector, pUC57
Transform and grow bacteria
isolate plasmids
Screen clones
Sequence to verify the gene
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Subcloning Email us for a quote
1. DNA fragment to be subcloned.
It can be a PCR product or a
plasmid carrying the target DNA
2. Subcloning vector or commercial
source to purchase the vector
Restriction digestion and ligation
Transformation and cell growth
Plasmid isolation
Screening and Selection of clones
DNA sequencing to verify the
insertion
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