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Molecular Biology

Site-directed Mutagenesis               catalog # MBS101
number of mutations 1 2 3
price ($) Email us for a quote Email us for a quote Email us for a quote
  • One mutation is one codon change on a gene. It also includes deletion or
  • The price is for insertion that is less than 2 kb. For > 2 kb, the price is 10% higher.
  • Bi-directional sequencing results to the insertion will be provide. Additional charge applies to full vector sequencing.
  • Customer provide: plasmid DNA
  • Turn around time is 2-3 weeks
Plasmid Preparation                   catalog# MBS102
Plasmid mini-prep Plasmid maxi-prep
Gene cloning and Subcloning                  catalog# MBS103
Gene cloning Email us for a quote
  • Customer provide:
The sequence of the gene to be cloned and the template source, such as a cDNA library
  • We perform:
Design and synthesize primers PCR and ligate PCR products into our standard vector, pUC57 Transform and grow bacteria isolate plasmids Screen clones Sequence to verify the gene
Subcloning Email us for a quote
  • Customer provide:
1. DNA fragment to be subcloned. It can be a PCR product or a plasmid carrying the target DNA
2. Subcloning vector or commercial source to purchase the vector
  • We perform:
Restriction digestion and ligation Transformation and cell growth Plasmid isolation Screening and Selection of clones DNA sequencing to verify the insertion