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Site-directed Mutagenesis catalog # MBS101
number of mutations	1	2	3
price ($)	Email us for a quote	Email us for a quote	Email us for a quote
One mutation is one codon change on a gene. It also includes deletion or The price is for insertion that is less than 2 kb. For > 2 kb, the price is 10% higher. Bi-directional sequencing results to the insertion will be provide. Additional charge applies to full vector sequencing. Customer provide: plasmid DNA Turn around time is 2-3 weeks

Plasmid Preparation catalog# MBS102
Plasmid mini-prep Minimal order: 96 preps Plasmid yield: 3-5 ug per prep Price without restriction digestion: Email us for a quote With restriction digestion: Email us for a quote Discount for large orders	Plasmid maxi-prep Minimal order: 24 preps Plasmid yield: 100 - 200 ug/prep Price: Email us for a quote Discount for large orders. Email us for a quote

Gene cloning and Subcloning catalog# MBS103
Gene cloning Email us for a quote Customer provide: The sequence of the gene to be cloned and the template source, such as a cDNA library We perform: Design and synthesize primers PCR and ligate PCR products into our standard vector, pUC57 Transform and grow bacteria isolate plasmids Screen clones Sequence to verify the gene	Subcloning Email us for a quote Customer provide: 1. DNA fragment to be subcloned. It can be a PCR product or a plasmid carrying the target DNA 2. Subcloning vector or commercial source to purchase the vector We perform: Restriction digestion and ligation Transformation and cell growth Plasmid isolation Screening and Selection of clones DNA sequencing to verify the insertion

Gene cloning and Subcloning catalog# MBS103

Gene cloning Email us for a quote

Customer provide:

The sequence of the gene to be cloned and the template source, such as a cDNA library

We perform:

Design and synthesize primers PCR and ligate PCR products into our standard vector, pUC57 Transform and grow bacteria isolate plasmids Screen clones Sequence to verify the gene

Subcloning Email us for a quote

Customer provide:

1. DNA fragment to be subcloned. It can be a PCR product or a plasmid carrying the target DNA
2. Subcloning vector or commercial source to purchase the vector

We perform:

Restriction digestion and ligation Transformation and cell growth Plasmid isolation Screening and Selection of clones DNA sequencing to verify the insertion