Home > Gene Synthesis
EZBiolab Inc.  All rights reserved
price
$0.69/bp
delivery time
<1 kb, 2-3 weeks
1-2 kb, 3-4 weeks
2-3 kb, 4-6 weeks
3-5 kb, 6-8 weeks
> 5kb, please ask
vector
pBluescript II SK
or pUC57
amount delivered
2-5 ug plasmid
(lyophilized powder)
quality control
in-vector sequencing (bi-directional)
guarantee
100% accuracy
EZBiolab Custom Gene Synthesis


















How to Order

email:       
service@ezbiolab.com

Web
click here

Fax:
download fax order form
(Gene sequence needs to
be submitted
electronically through
email or web order form)
$0.69/bp
Price of gene synthesis hits rock bottom
Simply inform us a sequence, we will send you the
gene cloned in a vector with 100% accuracy.
  • We provide free codon optimization service
  • All genes are cloned into our standard vectors (pBluesscript II
    SK or pUC57) through blunt end ligation. If you want a gene
    to be subcloned into a different vector, please add $295.00
    and allow 5-10 more days. You should provide us the
    subcloning vector or a commercial source to purchase the
    vector. In the later case, the cost of subcloning vector will be
    added to the final invoice.
  • Minimal order is 250 bp. Less than 250 bp will be charged at
    the price of 250 bp.
  • Sequencing result will be provided.
  • For difficult sequence, such as multiple repeating or high GC
    content (>70%), please ask for a quote.
  • Absolutely confidential
  • Shipping is $30.00 ($45 for international customers) For
    international shipment, shipping insurance that is 1% of total
    order will be added to the final invoice. This is the addition to
    the $45 shipping charge.
  • Shipping carrier is US post office express mail (globe express mail
    internationally). It is an overnight delivery service. For international
    delivery, it usually takes 2-4 days .Other delivery service such as
    Fedex, DHL or UPS is available at a higher price
Terms and Conditions
Why do you need a
synthetic gene?
At the price we are offering, it costs
less by ordering a synthetic gene
than PCR it yourself. More
importantly, it will save you a lot of
precious research time.Additionally,
gene synthesis provides some
unique applications impossible or
difficult to  be accomplished by PCR.
Below is a partial list:

  • codon optimization
  • multi-site mutagenesis
  • restriction sites
  • create a new gene
  • genes difficult to be
    obtained by PCR
  • more
What exactly will you receive?
The synthetic gene is inserted into a
pBluescript II or pUC57 vector
through blunt end ligation, usually
at Sma I site. What you receive is 2-5
ug such plasmid containing the
gene you have requested. You can
ask your gene being subcloned into
another vector, such as a protein
expression vector. Subclone fee is
$295.00.
download plasmid maps:
About codon optimization
It has been shown that simply place
the most frequently used codons
into a gene will not guarantee high
level of protein expression. GC
content, the secondary structure,
repeating sequences and some
other factors can also affect the
expression significantly.  Our codon
optimization algorism considers all
factors that can affect protein
expression and generate a
sequence optimized for protein
expression in host cells. Exactly to
say, this is sequence optimization
instead of codon optimization