Price of gene synthesis hits rock bottom
Simply inform us a sequence, we will send you the gene cloned in a vector with 100% accuracy.
Terms and Conditionsprice | Email us for a quote |
---|---|
delivery time | <1 kb, 2-3 weeks 1-2 kb, 3-4 weeks 2-3 kb, 4-6 weeks 3-5 kb, 6-8 weeks > 5kb, please ask |
vector | pBluescript II SK or pUC57 |
amount delivered | 2-5 ug plasmid (lyophilized powder) |
quality control | in-vector sequencing (bi-directional) |
guarantee | 100% accuracy |
At the price we are offering, it costs less by ordering a synthetic gene than PCR it yourself. More importantly, it will save you a lot of precious research time.Additionally, gene synthesis provides some unique applications impossible or difficult to be accomplished by PCR. Below is a partial list:
The synthetic gene is inserted into a
pBluescript II or pUC57 vector
through blunt end ligation, usually
at Sma I site. What you receive is 2-5
ug such plasmid containing the
gene you have requested. You can
ask your gene being subcloned into
another vector, such as a protein
expression vector. Subclone fee is
$295.00.
download plasmid maps:
It has been shown that simply place the most frequently used codons into a gene will not guarantee high level of protein expression. GC content, the secondary structure, repeating sequences and some other factors can also affect the expression significantly. Our codon optimization algorism considers all factors that can affect protein expression and generate a sequence optimized for protein expression in host cells. Exactly to say, this is sequence optimization instead of codon optimization