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.... Polyclonal Antibody : information
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Purity and length of peptide antigen
The purity of the peptide included in AB203 package is >70%. The
impurity of a HPLC purified peptide is mainly shorter peptides that
have little effect on specificity of antibodies raised. Peptide antigen
at higher purity is available at slightly higher price. Please see
package description for detail.
The length of a peptide epitope recognized by a antibody is about 7
residue long. A 15 residue peptide can contain multiple epitopes
due to the overlap of epitope sequences.
Conjugation of peptide antigens to a carrier protein, such as KLH
( Keyhole Limpet Hemocyanin) can significantly boost antigenitic power
of a peptide. The conjugation is usually done through the -SH group of a
Cys residue or -NH2 group. Conjugation through Cys's -SH group usually
generates stronger immuno-reaction from animals. This is because
its coupling efficiency is much higher. For this reason, we strongly
recommend you to add a Cys at the either end of a peptide antigen if
there is not an internal Cys residue in the peptide. Also, a Cys is required
in cross-linking peptide antigens to support matrix for affinity purification
of antibodies.
EZBiolab also provides conjugation to other carrier proteins, such as
OVA or BSA.
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peptide purity and length
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