....  Polyclonal Antibody: Protocol
Standard Protocol
week 0
pre-immu serum collection
Pre-bleed (~ 2 ml)
week 0
Primary injection
0.5 - 1.0  mg peptide-KLH emulsified with CFA injected
subcutaneously
~ week 2
1st boost
0.5 - 1.0  mg peptide-KLH emulsified with IFA injected
subcutaneously
~ week 4
2nd boost
0.5 - 1.0 mg peptide-KLH emulsified with IFA injected
subcutaneously
~ week 6
Serum collection
Bleed 1 ml per rabbit and Elisa to check titer
~ week 7
3rd boost
0.5 - 1.0 mg peptide-KLH emulsified with IFA injected
subcutaneously
~ week 9
Serum Collection
Bleed 1 ml per rabbit
~ week 10
ELISA, Serum collection and
shipping
Verify and terminate
Extended Protocol
( continue from week 9 )
~ week 10
ELISA and serum collection 1
~10 ml per rabbit
~ week 11
antigen injection
 
~ week 12
serum collection 2
~10 ml per rabbit
~ week 13
antigen injection
 
~ week 14
serum collection 3
~10 ml per rabbit
    Note for extented protocol:
    The protocol will be extended according to customers' request.
    Price:    $45.00 per extra serum collection per animal.  
    Animal maintenance: $1.00 per rabbit per day
    For serum collection more than 4 times ( including final termination),
    extra peptide synthesis and conjugation are required.

          
...


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    Polyclonal antibody protocol