Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. β-actin (gene name ACTB) is one of the two non-muscle cytoskeletal actins, molecular weight is 43 kDa. β-actin gene is often stably and constitutively expressed at high levels in most tissues and cells.
Proteins such as β-actin, involved in maintenance of basic cellular function, are often referred to as housekeeping proteins that are frequently used as loading controls for western blots and protein normalization.
A highly specific and sensitive rabbit polyclonal antibody against β-actin.
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human beta-actin.
WB 1:1000-2000, ICH 1:500-2000. Optimal dilution has to be determined by the user.
mouse, rat, human.
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5% BSA with 0.05% sodium azide. Antibody was purified by affinity chromatography.
store at 2-8℃ for 3 months, -20℃ for 1 year. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20℃) or for long-term storage (-80℃).
For research use only, not for use in diagnostic procedures.
Western blot analysis of β-Actin expression in Rat liver (1), HeLa (2), Sp/20 (3) and NIH/3T3 (4) whole cell lysates.
EZ-Agarose Mini Gel Cassette
1.0% or 2% agarose Mini format gel, 10 wells TBE buffer, EB dye # of gels: 10 Shelf life: 18 months Includes a buffer-dam opene