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Lentiviral shRNA (vshRNA) Service
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- Custom Preparation of vshRNA Lentiviral Particles
- Custom Construction of vshRNA Vectors
- Free shRNA Design
- TripleStrike Package: Gene Silence Guaranteed!
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siRNA can be delivered into cells by infection of the cells with Lentiviral particle carrying a siRNA
gene. The gene is then integrated into chromosome DNA of the host cells and is constantly expressed
to achieve long term gene silence (>30 days). Lentiviral particles can be transducted into a wide
variety of cells. The major advantage of Lentiviral shRNA over regular shRNA is that vshRNA can be
delivered into both dividing and non-dividing cells to achieve stable long term gene silence effect. For
more comparison of siRNA, shRNA and vshRNA, please click here
The procedure of vshRNA preparation is shown in following flow chart:
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Construction of
viral shRNA vector
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Transfection and
grow viral paricles
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Infect host cells and
silence the target gene
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Construction of a vshRNA vector and preparation of viral particles are two essential steps to achieve
gene silence by vshRNA. EZBiolab provide both of the services.
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1. vshRNA Vector Construction Service
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Custom Construction of Lentiviral shRNA Vector
This service is for the projects that already have a sequence of shRNA. If you need guaranteed results
from our design, please see our TripleStrike package.
Construction of vshRNA vector is to insert a synthetic DNA oligos (60-110 bp) into a vector. This
seemly simple experiment is much more troublesome than many expected for the following reasons:
- synthesis of long oligos ( >50mer) is prone to errors
- oligos for shRNAs often form hairpin structure, which is difficult to be cloned into a vector
- hairpin structure is difficult to be sequenced
If you already have a shRNA sequence , we can construct the vshRNA vector for you. This could save
you a lot of time and troubles to make the construction yourself. We provide:
- A collection of EZBiolab's Lentiviral vectors specifically designed for shRNA
- Multiple selections of report genes, promotors and antibiotic resistance
- Free design service
- Guaranteed sequence accuracy
- TripleStrike vshRNA package that guarantees to knockdown your gene (see below)
- Price: $425.00 per construction for 1-2 constructions.
(10% discount applies to order >2)
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TripleStrike Custom vshRNA Vector Construction Package
Guarantee to silence the target gene
Similar to our siRNA synthesis and regular shRNA vector construction services, our TripleStrike
Custom vshRNA Vector Construction package provides design and construction of three vshRNA
vectors targeting three different regions of a gene and a control shRNA vectors. Customer only need to
provide us a gene accession number ( gene bank ID) or a gene sequence. We guarantee you that this
gene will be silenced.
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Package Includes Design and Construction:
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1. Three shRNA expression vectors
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2. Negative control shRNA
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Customer Provide
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A gene bank ID (accessary number) or the sequence of a gene and a blank vector
(customer can also provide three shRNA sequences of their own design)
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Guarantee
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At least one of the three shRNAs will generate 70% silence effect at mRMA level
(no guarantee is offered for customer's own design)
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