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shRNA vector Construction
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A shRNA designed to target a specific gene can be delivered into cells through
a vector. The vector carries a DNA sequence that codes the shRNA. In cells,
the expressed shRNA is processed into siRNA and then blocks its target gene.
Comparing to chemically synthesized siRNA, shRNA has these features:
- Transfection efficiency can be monitored by report genes
- Transfected cells can be selected from non-transfected ones based on
- Experiments are operated at DNA level, which is much less accessible
- One time construction, unlimited supply.
For more comparison between siRNA and shRNA, click here
Construction of shRNA vector is to insert a synthetic DNA oligos (60-110 bp)
into a vector. This seemly simple experiment is much more troublesome than
many expected for the following reasons:
- synthesis of long oligos (>50mer) is prone to errors
- oligo for shRNAs often forms hairpin structure, which is difficult to
- hairpin structure is difficult to be sequenced
EZBiolab custom shRNA construction service is designed to release you from
this labour-intensive and time consuming work. Our service provides
- a large collection of EZBiolab's vectors specifically designed for shRNA
- multiple selections of report genes, promoters and antibiotic resistance
- free design service
- guaranteed sequence accuracy
- TripleStrike shRNA package that guarantees to knockdown your gene!
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TripleStrike Custom shRNA Construction Package
Silence Result Guaranteed
This all-inclusive package provides design and construction of three shRNA vectors
targeting three different regions of a gene and two control shRNA vectors. Customer
only need to provide us a gene accession number ( gene bank ID) or a gene sequence.
We guarantee you that this gene will be silenced by the package
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Package Includes Design and Construction
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1. Three shRNA expression vectors
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2. Positive control shRNA
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A gene bank ID (accessary number) or the sequence of a gene
(customer can also provide three shRNA sequences of their own design)
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At least one of the three shRNAs will generate 70% silence effect at mRMA level
(no guarantee is offered for customer's own design)
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shRNA Vector Construction Service
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shRNA vector of customer provided shRNA sequence
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Rsh-01
+ positive control shRNA vector (20 ug)
+ negative control shRNA vector (20 ug)
+ RT PCR primer for positive control (0.5 OD)
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shRNA vector of customer provided miRNA sequence
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Rm-01
+negative control miRNA vector (20 ug)
+RT PCT primer for posive control (0.5 OD)
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- Positive control: GAPDH.
- All constructed vectors are delivered at ultra pure grade and ready for transfection
- We provide free design service.
- For guaranteed knockdown, please see TripleStrike package
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Vector and Insertion Options
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- 25(stem) + 4 (loop) structure, for 25 bp target
- micro-30 miRNA
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