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Site-directed Mutagenesis catalog # MBS101
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- One mutation is one codon change on a gene. It also includes deletion or
insertion of one codon
- The price is for insertion that is less than 2 kb. For > 2 kb, the price is 10% higher.
- Bi-directional sequencing results to the insertion will be provide. Additional
charge applies to full vector sequencing.
- Customer provide: plasmid DNA
- Turn around time is 2-3 weeks
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Plasmid Preparation catalog# MBS102
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- Minimal order: 96 preps
- Plasmid yield: 3-5 ug per prep
- Price without restriction digestion:
- With restriction digestion:
- Discount for large orders
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- Minimal order: 24 preps
- Plasmid yield: 100 - 200 ug/prep
- Price: $37.50/prep
- Restriction digestion results
provided
- Discount for large orders. Please
email us for a quote
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Gene cloning and Subcloning catalog# MBS103
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The sequence of the gene to be
cloned and the template source,
such as a cDNA library
Design and synthesize primers
PCR and ligate PCR products into
our standard vector, pUC57
Transform and grow bacteria
isolate plasmids
Screen clones
Sequence to verify the gene
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1. DNA fragment to be subcloned.
It can be a PCR product or a
plasmid carrying the target DNA
2. Subcloning vector or commercial
source to purchase the vector
Restriction digestion and ligation
Transformation and cell growth
Plasmid isolation
Screening and Selection of clones
DNA sequencing to verify the
insertion
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- Contract research & production
Long term contract to outsource
your molecular biology works
Large scale DNA preparation,
DNA sequencing and oligo
synthesis
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