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His tag polyclonal antibody

  • Description

Background

His tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.

His tagging is the option of choice for purifying recombinant proteins in denaturing conditions because its mode of action is dependent only on the primary structure of proteins.

Description

A highly specific and sensitive rabbit polyclonal antibody against His tag.

Source

KLH-conjugated synthetic peptide encompassing a 6xHis sequence.

Applications

WB 1:1000-2000. Optimal dilution has to be determined by the user.

Applicable Species

The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins.

Specifications

Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5% BSA with 0.05% sodium azide. Antibody was purified by affinity chromatography.

Storage conditions

store at 2-8℃ for 3 months, -20℃ for 1 year. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20℃) or for long-term storage (-80℃).

Note

For research use only, not for use in diagnostic procedures.

Data

Western blot analysis of recombinant His tagged protein.

Introduction Catalog # Price  

EZ-Agarose Mini Gel Cassette

1.0% or 2% agarose Mini format gel, 10 wells TBE buffer, EB dye # of gels: 10 Shelf life: 18 months Includes a buffer-dam opene

E201

$69.95

Data not available (103)

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EZ-Agarose Mini Gel Cassette

1.0% or 2% agarose Mini format gel, 10 wells TBE buffer, EB dye # of gels: 10 Shelf life: 18 months Includes a buffer-dam opene

E201

$69.95

Data not available (103)

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'"> Data not available (103) ">
Order by Fax Order by Email

EZ-Agarose Mini Gel Cassette

1.0% or 2% agarose Mini format gel, 10 wells TBE buffer, EB dye # of gels: 10 Shelf life: 18 months Includes a buffer-dam opene

E201

$69.95

Data not available (103)

Return to Previous Page
'"> Data not available (103) ">
Order by Fax Order by Email

EZ-Agarose Mini Gel Cassette

1.0% or 2% agarose Mini format gel, 10 wells TBE buffer, EB dye # of gels: 10 Shelf life: 18 months Includes a buffer-dam opene

E201

$69.95

Data not available (103)

Return to Previous Page
'"> Data not available (103) ">
Order by Fax Order by Email

EZ-Agarose Mini Gel Cassette

1.0% or 2% agarose Mini format gel, 10 wells TBE buffer, EB dye # of gels: 10 Shelf life: 18 months Includes a buffer-dam opene

E201

$69.95

Data not available (103)

Return to Previous Page
'"> Data not available (103) ">
Order by Fax Order by Email