Glutathione-S-Transferase (GST) is an enzyme, which catalyzes the reaction of glutathione with a wide range of electrophilic substrates and therefore plays a role in the detoxification of potential alkylating agents. GST from Schistosoma japonicum, has been cloned in several expression vectors as a 27.5 kDa molecule, and used as a tag for expressing proteins of interest. These proteins are thus expressed as GST-fusion proteins. Antibodies to GST are useful in identifying the successful expression of these fusion proteins.
a mouse monoclonal antibody raised against Glutathione-S-Transferase.
WB 1:1000-2000, ELISA 1:500-2000. Optimal dilution has to be determined by the user.
GST from Schistosoma japonicum in GST fusion proteins.
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5% BSA with 0.05% sodium azide. Antibody was purified by affinity chromatography.
store at 2-8℃ for 3 months, -20℃ for 1 year. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20℃) or for long-term storage (-80℃).
For research use only, not for use in diagnostic procedures.
Western blot analysis of GST-tagged fusion proteins.
EZ-Agarose Mini Gel Cassette
1.0% or 2% agarose Mini format gel, 10 wells TBE buffer, EB dye # of gels: 10 Shelf life: 18 months Includes a buffer-dam opene